Fig. 5.
Effects of MPTP and WIN on microglial activation/infiltration. Saline or MPTP (4x 20 mg/kg, i.p.) were administered every 2 hr on day 0 and animals were sacrificed on day 3. Vehicle or cannabinoids were administered on days 1–3. (A) Representative microimages of the ventral midbrain immunostained for MAC-1 (scale bar = 100 μm). (B) Higher magnification image of the SNc immunostained for MAC-1 3 days post-MPTP treatment (scale bar = 25 μm). (C) Densitometry of MAC-1 expression in ventral midbrain following western blotting. *p<0.05 and **p<0.01 compared to saline-treated control (dashed line); ***p<0.001 compared to MPTP+vehicle (ANOVA followed by Bonferroni’s multiple comparisons post-test; n=3 per treatment).