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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Peptides. 2009 Jul 7;30(10):1882–1891. doi: 10.1016/j.peptides.2009.06.030

Table 1.

Comparison of Ratios of CCK9 to CCK8 from AtT-20 Cells Treated with Cathepsin L Inhibitor or PC1/3 Antisense.

Treatment Ratio CCK9/CCK8
None (control) 1.00 ± 0.06
CLIK-148 0.49 ± 0.15*
PC1/3 antisense 1.58 ± 0.14*

AtT-20 cells were treated with the specific cathepsin L inhibitor, CLIK-148 (24 hrs), or with PC1/3 antisense (by stable expression). At the end of the treatment period, secretion media was collected and subjected to HPLC analyses and CCK RIA (as shown in figure 5). The amounts of CCK8 and CCK9 were compared as ratios, with the ratio normalized to 1.00 for untreated control cells. Differences in ratios of CCK9/CCK8 were statistically significant, with p < 0.05 (by student's t-test, n = 4-6).