Figure 4.

Western blot of F1-ATPase beta subunit in subcellular fractions of HepG2 cells incubated in the presence or absence of enterostatin for 1 hour. 20μg protein from plasma membrane (PM), light mitochondrial fraction (MT) or cytosolic fraction (CYT) was loaded onto the gel. Marker assays showed the MT and PM fractions were free of cross contamination and neither MT or PM marker activity was evident in the cytosolic fraction..