Figure 5.
Effects of Dynamin 2 and Scamp 2 on insulin secretion from Beta-TC6 cells. Panel A shows knockdown of Dynamin 2 and Scamp2 by their respective siRNAs (D and S) compared to a control (C) scrambled siRNA. Panel B shows effects of siRNA to Scamp2 and Dynamin 2 on glucose stimulated insulin secretion and on the inhibitory response to enterostatin. Panel C shows that a transfected Dominant Negative Dyn2 (K44A) construct (DN) inhibits insulin secretion and blocks any further inhibitory effect of enterostatin on insulin secretion. Values represent Means ± SEMs for a minimum of 4 observations/group. *** p<0.001 compared to controls or groups as shown.
