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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: J Immunol. 2009 Jun 19;183(2):1197–1206. doi: 10.4049/jimmunol.0803883

Figure 2.

Figure 2

TTP is required for full IL-10-mediated inhibition of TNFα production. A, Reduction of LPS-induced TNFα cytokine production in cells pretreated with IL-10. BMDMs from WT and TTP−/− mice were treated with LPS, or pretreated overnight with IL-10 followed by stimulation with LPS (ILon/LPS) for indicated time points, or left untreated (w/o). Supernatants were collected and analyzed for TNFα cytokine levels by ELISA. Reduction of TNFα cytokine levels by IL-10 pretreatment (IL-10 o/n) relative (in %) to LPS-alone treatment (100%) is depicted. SDs (n=4) are indicated. B, Effect of simultaneous treatment with IL-10 and LPS on TNFα cytokine production performed as described in A. Relative amounts of TNFα cytokine secreted by LPS-treated WT and TTP−/− BMDMs compared to cells simultaneously treated with IL-10/LPS are shown. SDs (n=3) are indicated. C, Reduction of LPS-induced TNFα mRNA in cells pretreated with IL-10. WT-BMDMs and TTP-deficient BMDMs (TTP−/−) were treated 2 hours with LPS or pretreated overnight with IL-10 followed by LPS addition (IL-10/L) and analyzed for expression of TNFα by qRT-PCR, normalized to HPRT mRNA. Shown is reduction of TNFα mRNA levels after IL-10/LPS treatment in relation to the sample treated with LPS alone. SDs (n=3) are indicated.