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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Circulation. 2009 Aug 24;120(10):897–905. doi: 10.1161/CIRCULATIONAHA.109.853267

Figure 2. Systemic nitrite depletion after global ischemia and therapeutic repletion.

Figure 2

Whole blood (A) and plasma (B) nitrite levels (n=6) measured after 12 minutes of global ischemia and 5 minutes reperfusion are depleted in placebo vs. sham, and nitrite therapy increases levels with similar trends in brain (C). (D) A sample reductive chemiluminescence tracing measuring nitrite in heart 15 min after CPR. Peaks represent total tissue nitrite (first peak), S-nitrosothiols (second peak) and the mercury stable fraction (third peak; not visible). (D) Ischemia depletes heart nitrite in placebos and nitrite therapy significantly increases total levels and (E) S-nitrosothiols (n=7). Values denoted as means±SEM analyzed by ANOVA; *, p<0.01 (placebo vs. sham); †, p<0.01 and ‡, p<0.05 (nitrite vs. placebo).