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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Circulation. 2009 Aug 24;120(10):897–905. doi: 10.1161/CIRCULATIONAHA.109.853267

Figure 6. Nitrite therapy effects on mitochondrial function.

Figure 6

Heart mitochondrial respiration time course where zero represents CPR start and shaded area indicates arrest. (A) Inhibition of pyruvate (complex I) mediated respiration was present at 5 (n=8) and 15 minutes (n=4) post-CPR but reversed by 1 hour. (B) Succinate (complex II) mediated respiration rates did not differ (n ≥ 4 for each time). Inset: sample traces of mitochondrial oxygen consumption 5 minutes post-CPR. (C) Complex I activity measured in sub-mitochondrial particles by NADH oxidation. Compared to placebo, nitrite therapy significantly reduced complex I activity 5 minutes post-CPR (n=7) which was reversed by 60 minutes. Inset: sample tracing 5 minutes post-CPR. Values denoted as means±SEM, analyzed at each time by paired t-test; *, p<0.01; †, p=0.021; ‡, p=0.014.