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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Eur J Immunol. 2008 Dec;38(12):3450–3458. doi: 10.1002/eji.200838640

Fig. 5. Diminished IL-17 and IgG2a autoantibody production were associated with reduced arthritis in APRIL−/− mice.

Fig. 5

A, C, E, F. Inguinal LN cells, isolated one week after a chick CII/CFA immunization, were cultured at 2×106/ml in the absence or presence of denatured chick CII (100μg/ml) or anti-CD3 (2.5μg/ml) for 4 days. Cytokine production in supernatant was detected by ELISA. *, p=0.0291; n=7; unpaired t test. Data from 4 independent experiments (n=1–2 each) were combined. B, D. Splenocytes, isolated on day 17 after OVA/CFA immunization on day 0 and OVA/IFA immunization on day 14, were cultured at 5×106/ml in the absence or presence of OVA (100μg/ml) or anti-CD3 (2.5μg/ml) for 3 days. Cytokine production in supernatant was detected by ELISA. n=6, from the mice in Fig. 2. H, I. with serum IgG1 and IgG2a tested. G – I. Correlation between each two of the cytokine production of IL-17, IL-6 and TNF-α. n=14, linear regression: r2=0.7155, p=0.0001 (G), r2=0.5585, p=0.0021 (H), r2=0.8425, p<0.0001 (I), J – L. Serum antibody titers to mouse CII on day 34, detected by ELISA. The serum titer shown was within the linear range of antibody dilution; n=5–6 (n=3 for non-arthritis APRIL+/+ mice). *, p<0.05; **, p<0.01; ***, p<0.001; two-way ANOVA followed by Bonferroni post-tests.