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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: Chem Res Toxicol. 2009 Aug 17;22(8):1427–1434. doi: 10.1021/tx900110n

Figure 5. Regeneration of dithiolethiones by O2 subsequent to their reduction by NaBH4.

Figure 5

(A) HPLC chromatogram of 2. (B) HPLC chromatogram of 2 subsequent to reduction, in an argon atmosphere, with excess NaBH4 for 1h in H2O containing 20% ethanol by volume. (C) HPLC chromatogram of the material in panel (B) after, quenching with acetic acid, adjusting to pH 7.4 with phosphate buffer and bubbling under a stream of O2 for 10 min. Chromatograms D-F are a similar sequence with 3, but the reduction time was 40 min. For further details see Experimental and Results.