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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: Cell Host Microbe. 2009 Jan 22;5(1):13–21. doi: 10.1016/j.chom.2008.12.007

Figure 2. Two-Photon Fluorophore Excitation Is Limited to the Focal Plane.

Figure 2

During multiphoton excitation, short, high-intensity pulses are delivered to the tissue or cells being examined. Because two photons must be absorbed within an attosecond to accomplish fluorophore excitation, this is only accomplished within the focal plane. Cells outside of the focal plane are not excited, and a traditional confocal pinhole is eliminated during this mode of microscopy.