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. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Nature. 2009 Aug 23;461(7261):230–235. doi: 10.1038/nature08283

Figure 2. hTERT and RMRP exhibit RdRP activity.

Figure 2

a, Predicted RNA products produced by RdRP or TT activity. b, RNA products produced by the RdRP activity derived from recombinant hTERT and RMRP. c,d Treatment of RNA products with RNase T1 (c) or bacterial RNase III (d). e, RdRP assay performed in the presence of ribonucleotides (middle) or in the absence of adenine (left lane) or guanine (right lane) ribonucleotides. A and G are present within the first 5 nt of the predicted complementary strand of RMRP. f, DN-hTERT binds RMRP but lacks RdRP activity. TERT immune complexes were isolated from 293T cells expressing FLAG-tagged hTERT or FLAG-tagged DN hTERT. RdRP activity is shown in lower panel. g, Northern blotting to detect complementary sequence of RMRP. h, Time course of RdRP activity. i, RNA products produced by recombinant hTERT and truncation mutants of RMRP transcribed in vitro. Faint signals at the 200 nt, 120 nt and 60 nt are hTERT TT products. j, RNA products produced by the RdRP activity derived from recombinant hTERT or DN hTERT and total RNA. A limited pool of RNAs serve a templates for RdRP activity.