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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Pharm Sci. 2009 Nov;98(11):4170–4190. doi: 10.1002/jps.21723

Figure 9.

Figure 9

Figure 9 is the change in abacavir cellular accumulation and cell-free equilibrium dialysis free fraction with increasing concentrations of Pluronic P85. The left y-axis depicts cellular accumulation in MDCK WT and MDR1 cells in the presence (● -WT;◆ -MDR1) and absence (○ -WT;◇ -MDR1; mean ± S.D., n=3) of Pluronic P85. Plot also depicts the change in free fraction of abacavir with change in Pluronic P85 concentration (right y-axis) determined experimentally by equilibrium dialysis (∎ -P85,□ - no P85; n=4). *; statistically significant decrease in saquinavir accumulation in WT cells compared to 0.03% w/w P85 treatment (p<0.05). #; statistically significant decrease in saquinavir accumulation in MDR1 cells compared to 0.03% w/w P85 treatment (p<0.05). The effect of Pluronic P85 on mannitol cellular accumulation in MDCK WT and MDR1 cells is also depicted on the plot (▔ -WT; x -MDR1; mean ± S.D., n=9). The horizontal dotted line represents a free fraction of 1 and the vertical dashed line represents the critical micellar concentration of Pluronic P85.