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. 2009 Jun 26;284(34):22544–22548. doi: 10.1074/jbc.M109.014399

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Interaction between Dvl and PI4KIIα. A and B, Dvl1-Flag and PI4KIIα-HA or GSK3β-HA were coexpressed in HEK293T cells for 24 h, and PI4KIIα-HA or GSK3β-HA was immunoprecipitated with an anti-HA (A) or anti-Flag (B) antibody. The immunocomplexes were detected by indicated antibodies. GSK3β-HA was used as a control. *, Ig. C, purified His-tagged PI4KIIα was incubated with GST or Dvl1-GST proteins bound on glutathione beads for 3 h at 4 °C. After washing, proteins associated with beads were analyzed by Western blotting using anti-His or anti-GST antibody. D, pulldown of His-tagged Dvl1 by GST or PI4KIIα-K-GST proteins was detected by Western blotting using anti-His or anti-GST antibody. PI4KIIα-K lacks the N-terminal 92 amino acids.