FIGURE 5.
Interactions of Dvl, PI4KIIα and PIP5KIβ. A, immunoprecipitation of Dvl-Flag in HEK293T cells overexpressing PI4KIIα and/or PIP5KIβ. B, coimmunoprecipitation of PI4KIIα and PIP5KIβ in transfected HEK293T cells. ΔN-β-catenin-HA was used as a control. C, effect of Wnt3a on Dvl, PIP5KIβ and PI4KIIα complex formation. HEK293T cells stably expressing PI4KIIα-HA7 (4K), whose expression level is lower than that of endogenous PI4KIIα as detected by an anti-PI4KIIα antibody, were treated with or without 50 ng/ml Wnt3a protein for 30 min. Immunoprecipitation was carried out using an anti-HA antibody. HEK is the parental cell. D, hypothetical model depicting the regulation of PtdIns(4,5)P2 production by Wnt3a through Fz, Dvl and phosphatidylinositol kinases.