TABLE 1.
Characteristics of Hsp90 linker variants
For viability, see also Fig. 1C. The chemical stability was determined by urea-induced unfolding transitions. kcat and Km values were determined using the regenerative ATPase assay. All methods were used as described under “Materials and Methods.”
| Hsp90 wt | Hsp90 Δ211–242 | Hsp90 Δ211–259 | Hsp90 Δ211–263 | Hsp90 Δ211–266 | Hsp90 Δ211–272 | |
|---|---|---|---|---|---|---|
| Viability (20 °C) | + | + | + | + | - | - |
| Chemical stability (urea) [m] | 4.9 ± 0.1 | 4.9 ± 0.1 | 4.7 ± 0.1 | 4.9 ± 0.1 | 4.8 ± 0.1 | 5.1 ± 0.1 |
| ATPase kcat (min−1) | 0.5 | 0.6 | 0.6 | 0.3 | 0.2 | 0.1 |
| Km (ATP) (μm) | 320 ± 61 | 260 ± 55 | 300 ± 84 | 337 ± 92 | 406 ± 76 | 311 ± 64 |