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Characteristics of supplemented linker constructs
The non-viable linker deletions Hsp90 Δ211–266 and Hsp90 Δ211–272 were characterized with the respect to the ATPase rate, Km value, and stimulation by the co-factor Aha1 using the regenerative ATPase assay.
| Hsp90 Δ211–266SUP | Hsp90 Δ211–272SUP | |
|---|---|---|
| ATPase | ||
| kcat (min−1) | 0.7 | 0.3 |
| Km (μm) | 293 ± 120 | 313 ± 98 |
| Aha1 activation | + | + |
| ATPase stimulation factor | 14.5 | 5.2 |
| KD (μm) | 2.0 ± 1.5 | 1.8 ± 1.2 |
