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. 2009 Jun 29;284(34):22580–22589. doi: 10.1074/jbc.M109.027318

FIGURE 1.

FIGURE 1.

HFE2 and neogenin expression profiles in isolated rat liver cells. A, qRT-PCR analysis of HFE2 mRNA in isolated rat liver hepatocytes (Hep, n = 5), Kupffer cells (KC, n = 7), sinusoidal endothelial cells (SEC, n = 4), and hepatic stellate cells (HSC, n = 6). Results are expressed as the amounts relative to GAPDH. The mean values and the S.D. for each cell population are presented. B, qRT-PCR analysis of neogenin mRNA in isolated rat liver cell populations. The analysis was performed as described in A. C, Western blot analysis of HJV and Neo proteins in isolated rat liver cells. Cell extract protein (250 μg) was separated in SDS-PAGE under reducing conditions. Equal protein loading was confirmed by Ponceau S staining of the membrane (not shown). Membranes were probed with antibodies against HJV and neogenin. Cell lysate from HEK293 cells stably expressing both HJV and neogenin (HEK) was included as a positive control. Experiments were performed using two different sets of isolated cells and showed consistent results.