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. 2009 Jun 17;284(34):22641–22648. doi: 10.1074/jbc.M109.022400

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effect of MRAP on MC2 receptor homodimerization. Live CHO cells expressing HA-MC2 receptor-YFP-F1 and HA-MC2 receptor-YFP-F2 without (A) or with (B) MRAP-V5. YFP fluorescence is shown in green (left panel). Surface MRAP in the same cells detected with anti-V5 antibody and secondary anti-mouse Alexa546 is shown in red (right panel). C, average YFP fluorescence intensity was measured in five or more randomly selected fields. All successfully transfected cells, identified by staining with anti-V5 antibody, were analyzed (25–88 cells/condition). YFP fluorescence reports the formation of receptor homodimers. Shown are mean ± S.E. in relative fluorescence units (RFU). D, total expression of HA-MC5 receptor-YFP-F1 and HA-MC5 receptor-YFP-F2 without or with MRAP-V5 measured by ELISA in permeabilized cells using anti-HA antibody.