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. 2009 Jun 17;284(34):22641–22648. doi: 10.1074/jbc.M109.022400

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Effect of MRAP on MC5 receptor homodimerization measured by bimolecular fluorescence complementation. A and B, live CHO cells expressing HA-MC5 receptor-YFP-F1 and HA-MC5 receptor-YFP-F2 without (A) or with (B) MRAP-V5. YFP fluorescence is shown in green (left panel). Surface MRAP in the same cells detected with anti-V5 antibody and secondary anti-mouse Alexa546 is shown in red (right panel). C, average YFP fluorescence intensity in relative fluorescence units (RFU). D, total expression of HA-MC5 receptor-YFP-F1 and HA-MC5 receptor-YFP-F2 without or with MRAP-V5. YFP fluorescence and total expression of receptors was quantified as described in the legend to Fig. 4.