Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 17;284(34):22641–22648. doi: 10.1074/jbc.M109.022400

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Effect of MRAP on MC5 receptor homodimerization measured by co-immunoprecipitation. A and B, cells expressing HA-MC5 receptor and V5-MC5 receptor without (A) or with (B) MRAP were lysed, and MC5 receptor was immunoprecipitated with anti-HA or anti-V5 antibody. Proteins were resolved by SDS-PAGE, and HA-MC5 receptor was detected with anti-HA antibody. *, IgG heavy chain. C, quantitative analysis of HA-MC5 receptor and V5-MC5 receptor co-immunoprecipitation in the presence or absence of MRAP was performed as described under “Experimental Procedures.” It is expected that only a fraction of MC5 receptor dimers will have HA/V5 tags and be visualized, whereas others will have HA/HA or V5/V5 tags. The mean ± S.E. values from three separate experiments are shown. *, p < 0.01 compared with control.