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. 2009 Jun 17;284(34):22641–22648. doi: 10.1074/jbc.M109.022400

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Regions of MRAP required for regulation of MC5 receptor homodimerization and trafficking. A, surface HA-MC5 receptor was measured by ELISA on non-permeabilized CHO cells transfected with HA-MC5 receptor alone or with RAMP3 or the noted MRAPs. The bar graph represents the mean ± S.E. of three separate experiments, each performed in duplicate or triplicate, for surface expression of MC5 receptor normalized to control expression in the presence of RAMP3. B, Western blot analysis of RAMP3, MRAP, and MRAP mutants or MRAP2 expression in cell lysates from parallel dishes in the experiment depicted in A. All of the accessory proteins were tagged with an amino-terminal V5 epitope and made in the same pCI-Neo vector. C, YFP fluorescence intensity in live CHO cells expressing HA-MC5 receptor-YFP-F1 and HA-MC5 receptor-YFP-F2 with V5-tagged RAMP3 or the noted MRAPs, analyzed as described in the legend to Fig. 4. Sequences of MRAP mutants have been reported previously (3, 4). *, p < 0.05 compared with control.