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. 2009 Jun 25;284(34):22672–22679. doi: 10.1074/jbc.M109.028001

FIGURE 2.

FIGURE 2.

C-terminal TM truncation reduces HO-1 activity and abolishes oligomerization. A, confocal images of HEK293 cells 24 h after co-transfection with pDsRed-ER and plasmid coding for YFP-HO-1-FL or YFP-HO-1-CΔ24. The ER and HO-1 images were obtained using the ER or YFP filter set, respectively. B, HO activities in cells transfected with YFP-HO-1-FL or YFP-HO-1-CΔ24 plasmids were determined. Data are the mean ± S.E. of four independent experiments. *, p < 0.01 versus cells overexpressing YFP-HO-1-FL. C, epi-fluorescent images of HEK293 cells 24 h after co-transfection with plasmids coding for CFP and YFP (CFP+YFP), CFP-HO-1-FL and YFP-HO-1-FL (HO-1-FL), or CFP-HO-1-CΔ24 and YFP-HO-1-CΔ24 (HO-1-CΔ24). Images were obtained using the CFP, YFP, or FRET cube as indicated. D, averaged FR and FE calculated using Equations 1 and 2. The number of cells quantified in each group is shown in parenthesis. ***, p < 0.005 versus control cells transfected with CFP + YFP. E, plot of FR versus CFP cube intensity per area from cells coexpressing the indicated proteins.