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. 2009 Jun 25;284(34):22672–22679. doi: 10.1074/jbc.M109.028001

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — The W270N mutation decreases HO-1 oligomerization. A, epi-fluorescent images of HEK293 cells 24 h after transfection with plasmids coding for CFP-HO-1-FL and YFP-HO-1-FL (HO-1-FL), CFP-HO-1-W270A and YFP-HO-1-W270A (HO-1-W270A), or CFP-HO-1-W270N and YFP-HO-1-W270N (HO-1-W270N). The granular ER is indicated by the stars in the insets and the perinuclear ER by the arrows. B, average FR and FE values in the granular and perinuclear ER or the cytoplasm. The number of cells quantified in each group is shown in parentheses. *, p < 0.05; ***, p < 0.005 versus the corresponding compartment in cells expressing wild type HO-1. †, p < 0.05 versus the granular ER of cells expressing HO-W270N.