FIGURE 2.

Protein two-dimensional ¹⁵N,¹H-HSQC spectra recorded in the presence of 12.9 mm DPC (red contours) or without addition of DPC (blue contours). Backbone ¹⁵N-¹H resonances of selected residues in the hydrophobic polypeptide segment (see Fig. 1) are labeled with the one-letter amino acid code and the positions in the sequence. The dotted lines connect corresponding peaks in the spectra recorded in the presence and absence of DPC. A, mPrP-(90–231); B, mPrP[P105L]-(91–231); and C, mPrP[A117V]-(90–231).