FIGURE 3.

Histograms of the ¹⁵N chemical shift changes induced by the addition of 6.8 mm DPC for wild-type mPrP-(90–231) and eight variants thereof. The data are plotted versus the residue numbers. At the top, the locations of the regular secondary structure elements in mPrP-(90–231) are shown. Δδ(¹⁵N) is the difference between the ¹⁵N chemical shifts in the absence of DPC and in the presence of 6.8 mm DPC. The locations of the amino acid substitutions in the variant proteins are indicated by asterisks. A, mPrP-(90–231); B, mPrP[M129V]-(91–231); C, mPrP[P102L]-(91–231); D, mPrP[P105L]-(91–231); E, mPrP[P105L,M129V]-(91–231); F, mPrP[A117V]-(90–231); G, mPrP[A117V,M129V]-(90–231); H, mPrP[A113V,A115V,A118V]-(90–231); I, mPrP[K110I,H110I]-(90–231).