FIGURE 2.

Transcriptional repression of different deletion mutants of Prdx6 gene promoter linked to CAT in Prdx6^+/+ and Prdx6^−/− cells. The top drawing illustrates the putative transcription-binding elements in the Prdx6 promoter. Construct A consists of two NF-κB sites (NF-κB-1 and NF-κB-2) and two repressive Smad3-binding elements (RSBE-1 and RSBE-2). Two deletion mutants were generated: Construct B with one NF-κB and two RSBE sites and Construct C with one RSBE site only. Cells were transiently transfected with Prdx6-CAT Construct A, B, or C. After 72 h, protein was extracted, and CAT activity was measured. The transfection efficiencies were normalized using a plasmid secreted alkaline phosphatase basic vector. The data represent the mean ± S.D. from three independent experiments.