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. 2009 Jun 24;284(34):22758–22772. doi: 10.1074/jbc.M109.016071

FIGURE 3.

FIGURE 3.

A, TGFβ1-induced repression of Prdx6 gene transcription in Prdx6+/+ cells as seen in Prdx6−/−. The cells were transiently transfected with Prdx6-CAT Construct B or C containing an RSBE site(s) as in Fig. 2. Prdx6+/+ cells (striped bars) were treated with TGFβ1 at a concentration of 2 ng/ml for 3 days, and Prdx6−/− cells and untreated Prdx6+/+ cells served as control. CAT activity of these constructs in Prdx6−/− or TGFβ1-treated or -untreated Prdx6+/+ cells was compared. Results are mean ± S.D. from three experiments. B, TGFβ-neutralizing antibody reduced the repression of Prdx6 transcription in Prdx6−/− cells. Cells were transiently transfected with either Construct B or C, and TGFβ-neutralizing antibody (R&D Systems) was added at a concentration of 5 μg/ml for 3 days followed by CAT activity determination. Striped bars indicate untreated control, and dotted bars show cells treated with TGFβ1-neutralizing antibody.