FIGURE 5.

Point mutation at RSBE site(s) of the Prdx6 promoter abrogated repression of Prdx6 transcription in Prdx6^−/− cells. Left, schematic illustration of wild type (Constructs B and C having RSBE/TIE) and mutated (RSBE/TIE disrupted using site-directed mutagenesis) Prdx6 promoter constructs. Right, CAT activity of engineered wild type (black bars) and mutant (gray, light gray, and open bars) promoter constructs of Prdx6 was assessed for their transcriptional activity in Prdx6^−/− and Prdx6^+/+ cells. All data were presented as the mean ± S.D. from at least three independent experiments.