FIGURE 6.

Klf4-induced growth arrest is not dependent on RARα. A, Am80 or Ro 41-5253 was given orally to pAd- and pAd-Klf4-infected animals that were subjected or not to vascular injury. Three days later, the injured arteries were harvested and analyzed by Western blotting using the indicated antibodies. β-Actin was used as a loading control. The blots are representative of three independent animals. B, Am80 or Ro 41-5253 was given orally to pAd- and pAd-Klf4-infected animals that were subjected to balloon injury. Fourteen days later, arterial sections were subjected to histological analysis. Neointimal formation was evaluated by calculating the I/M ratio as indicated in Fig. 1A. *, p < 0.05 versus model group (n = 6 in each group).