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. 2003 Nov 15;31(22):6428–6434. doi: 10.1093/nar/gkg875

Figure 1.

Figure 1

Construction of 50mer deoxyoligonucleotide containing a single B[c]PhDE-dA adduct. B[c]PhDE-containing 21mer (oligonucleotide a) was ligated with oligonucleotide b after they were annealed to a complementary 50mer (oligonucleotide c). The ligated oligomer was then elongated to 50 nucleotides using oligonucleotide c as the template. The 50mer homoduplex (A-T) and heteroduplex containing an A-C mismatch, a 2-nt insertion/deletion (ID) mispair, or a 4-nt ID mispair were also constructed using similar procedures. The star represents B[c]PhDE adduct.