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B[c]PhDE-induced apoptosis in mouse small intestine. Mice (wild type, Msh2–/– or Mlh1–/–) were injected with B[c]PhDE (0.2 µmol/kg) and sacrificed at hour 0, 3, 6, 12 or 24 after injection. Paraffin sections of small intestines were analyzed for apoptosis by ApopTag Peroxidase Kits (Intergen Co., Purchase, NY). For each point, a minimum of three mice were used and 50 half-crypts were scored under a microscopy.
