FIGURE 1.

hYVH1 protects cells from oxidative stress. HeLa cells transiently transfected with either empty vector (Control) or hYVH1 and were treated with H₂O₂ (100, 250, or 500 μm) for 1 h at 37 °C. A, cell viability was detected using fluorescein isothiocyanate-conjugated annexin V as per the manufacturer's protocol, and total cell number was determined using Hoechst 33342 dye. B, graphic representation from three independent experiments expressing cell death as a percentage of total cell number determined using fluorescein isothiocyanate-conjugated annexin V and Hoechst 33342 nuclear stain. Error bars, S.D.