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The state of the gated C-terminal ring of PAN is substrate-dependent. Density at the C-terminal ring of PAN was analyzed by projecting a 2 nm-thick section at the position, shown in A. B, slices of the gate region in the presence of ATPγS or ADP or in the absence of nucleotide. C, when PAN is incubated with substrate (YFP-SsrA) and ATP, the C-terminal ring opens.
