TABLE 1.

Activation energy of the TS reaction and hydrolysis of F₁(βE190D) and wild-type F₁

Activation energy was calculated by the linear fitting of the Arrhenius plots of ATPase and the rotational rate as shown in Fig. 1B or by the comparison of rate constants at 18 and 28 °C determined from the dwell time histograms shown in Fig. 2, B and C, for βE190D and Fig. 4, C and D, for βE190D(hybrid).

Reaction	F1	Activation energy
		kJ/mol
TS	βE190D	86 (ATPase), 82 (rotation rate), 78 (dwell time)
	βE190D(hybrid)	62 (dwell time)
Hydrolysis	βE190D	11 (ATPase), 18 (rotation rate), 8.3 (dwell time)
	βE190D(hybrid)	16 (dwell time)
TS	Wild type	97a (ATPase), 98a (rotation rate)
Hydrolysis (ATPγS)	Wild type	2.4b (dwell time)

^a Determined from the temperature dependence of the maximum ATPase published in Watanabe et al. (21).

^b Determined by a comparison of the hydrolysis rates at 4 °C (21) and at room temperature (11).