Fig. 3.
Voltage dependence of the area-specific membrane capacitance increment (ΔCLI) induced in HEK cells by the adsorbed lipophilic anions WO− (a), WW− (b), DPA− (c) and TPB− (d). Membrane capacitance was measured by whole-cell patch clamp. The plotted ΔCLI data (symbols) were calculated by subtracting the capacitance values of control cells from those of cells treated with various anion concentrations: 5 μm (open circles), 20 μm (open squares) and 50 μm (filled circles). Curves are least-square fits of the first derivative of the Boltzmann function (equation 1). The fitted parameters are summarized in Table 1