Table 4.
The membrane capacitance increments (ΔCLI), translocation rate constants (ki) of mobile charges and cytosolic properties of HEK293 cells (σi and εi) derived from the ROT spectra in the presence of various concentrations of lipophilic anions
| Lipophilic anion | Concentration (μm) | ΔCLI (mF/m2) | ki [1/(s * 103)] | σi (mS/m) | εi/ε0 | n |
|---|---|---|---|---|---|---|
| Isotonic mediaa | ||||||
| WO− | 5 | 5.8 ± 0.9 | 540 ± 59 | 541 ± 66 | 112 ± 17 | 4 |
| WO− | 10 | 8.8 ± 1.0 | 451 ± 13 | 839 ± 64 | 175 ± 28 | 6 |
| WO− | 20 | 21.1 ± 4.1 | 290 ± 59 | 631 ± 56 | 168 ± 22 | 16 |
| WO− | 50 | 35.3 ± 4.8 | 163 ± 22 | 609 ± 40 | 180 ± 32 | 10 |
| WW− | 5 | 48.0 ± 4.4 | 423 ± 26 | 733 ± 139 | 195 ± 27 | 4 |
| WW− | 10 | 60.7 ± 3.6 | 241 ± 36 | 448 ± 45 | 106 ± 10 | 10 |
| WW− | 20 | 80.1 ± 9.0 | 285 ± 51 | 725 ± 17 | 222 ± 37 | 8 |
| WW− | 50 | 69.1 ± 3.5 | 185 ± 39 | 528 ± 76 | 126 ± 15 | 8 |
| Hypotonic mediaa | ||||||
| WO− | 20 | 33.0 ± 6.0 | 203 ± 21 | 470 ± 33 | 136 ± 24 | 12 |
| WW− | 50 | 78.0 ± 10.7 | 268 ± 27 | 496 ± 63 | 189 ± 33 | 10 |
| DPA− | 10 | 24.6 ± 2.5 | 20.2 ± 2.3 | 370 ± 30 | 153 ± 21 | 9 |
aIso- and hypotonic media of osmolalities of 300 and 100 mOsm, respectively, were used