Table 1.

Composition of artificial seawater, mineral solution, trace solution, and vitamin solution

Artificial seawater or solution	Composition
Artificial seawater	27.5 g NaCl, 10.7 g MgCl2 · 6 H2O, 4.1 g MgSO4 · 7 H2O, 0.66 g CaCl2 · 2 H2O, 1.02 g KCl, 1,000 mL distilled water
Mineral solution	555 mg K2HPO4, 50 mg Na2MoO4, 750 mg Na2S2O5, 29 mg FeCl3 · 6 H2O, 1,000 mL distilled water
Trace element solution	5.2 g EDTA, 1.5 g FeCl2 · 4 H2O, 0.07 g ZnCl2, 0.1 g MnCl2 · 4 H2O, 0.062 g H3BO4, 0.19 g CoCl2 · 6 H2O, 0.017 g CuCl2 ·2 H2O, 0.024 g NiCl2 ·6 H2O, 0.036 g Na2MoO4 · 2 H2O, 1,000 mL distilled water
Vitamin stock solutiona	1 mg B12, 1 mg Inositol, 1 mg Biotin, 1 mg Folic Acid, 10 mg PABA, 100 mg Nicotinic Acid, 100 mg d-Pantothenate, 200 mg Thiamine (each vitamin was dissolved in 10 mL distilled water)

^aFor the final vitamin solution, 1 mL of each vitamin stock solution was added to 100 mL (final volume) of distilled water