Table 2.

Composition of agar media

Agar mediaa	Composition
Bottom agar medium	180 mL artificial seawater, 1.35 g agar
Top agar mediumb	1) 240 mL artificial seawater, 4.32 g NaCl
	2) 96 mL distilled water, 0.9 g agar
	3) 24 mL mineral solution, 0.36 mL trace solution

^aEach medium was autoclaved

^bThe three components of the top agar medium were combined after they were autoclaved