Table 1.
Ignicoccus hospitalis proteins: biophysical and biochemical data
| Protein ID | Identification | Subcellular localizationa | No of amino acids | Mr in kDa (predicted) | Mr in kDa (experimentally) | Theoretical pI | Mascot score/sequence coverage (%) | SP/TMH (Phobius) | TMH (TMHMM) | TMH (SOSUI) | Fig./Lane |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Igni_0096 | MS-MS/MS | Cyt | 558 | 60.5653 | 60 | 5.37 | 101/29 | −/− | 0 | 0 | 1/4 |
| Igni_0139 | MS-MS/MS | MA (P) | 429 | 47.4129 | 55 | 8.89 | 245/51 | +/− | 0 | 1 | 2/4 |
| Igni_0235 | MS-MS/MS | M | 250 | 28.9785 | 25 | 9.50 | 91/12 | −/3 | 3 | 3 | 1b |
| Igni_0459 | Edmanb MS-MS/MS | Cyt | 234 | 26.7110 | 50/25/15 | 6.19 | 361/80 | −/− | 0 | 0 | 1/2, 2/2 and 5 |
| Igni_0608 | MS-MS/MS | Cyt | 343 | 37.7728 | 38 | 6.17 | 269/58 | −/− | 0 | 0 | 2/4 |
| Igni_0642 | MS-MS/MS | Cyt | 187 | 21.3518 | 19 | 8.34 | 72/53 | −/− | 0 | 0 | 2/2 |
| Igni_0670 | Edmanb | E | 310 | 33.2847 | 33 | 5.43 | – | −/1 | 2 | 3 | 2/2 |
| Igni_0679 | MS-MS/MS | MA | 471 | 52.2679 | 64/69 | 5.58 | 169/47 | −/− | 0 | 0 | 2/1 |
| Igni_0897 | MS-MS/MS | Cyt | 541 | 58.8272 | 58 | 5.74 | 357/48 | −/− | 0 | 0 | 1/4 |
| Igni_0899 | Edmanb MS-MS/MS | Cyt | 367 | 41.0202 | 40 | 6.31 | 293/70 | +/− | 0 | 1 | 1/1, 2/3 |
| Igni_0985 | Edmanb | Cyt or M | 138 | 15.4276 | 60 | 5.34 | – | +/3 | 3 | 3 | 1/2 |
| Igni_1007 | MS-MS/MS | Cyt | 414 | 44.9009 | 48 | 5.35 | 90/29 | −/− | 0 | 0 | 2/1 |
| Igni_1080 | MS-MS/MS | MA | 208 | 23.7543 | 25 | 5.21 | 94/37 | −/− | 0 | 0 | 1b |
| Igni_1150 | MS-MS/MS | Cyt | 442 | 49.2645 | 50 | 9.17 | 267/61 | −/− | 0 | 0 | 2/5 |
| Igni_1201 | Edmanb MS-MS/MS | MA (P) | 784 | 87.7122 | 53 | 5.19 | 223/29 | +/− | 0 | 1 | 2/3 |
| Igni_1266 | Edmanb | M | 85 | 8.3558 | 150/50/45 | 8.40 | – | −/1 | 1 | 1 | 1/7 and 8 |
| Igni_1323 | Edmanb | M | 92 | 9.9489 | 10 | 8.06 | – | +/2 | 3 | 3 | n. s. |
| Igni_1348 | MS-MS/MS | Cyt | 124 | 14.0931 | 14 | 6.10 | 173/82 | −/− | 0 | 0 | 2/5 |
| Igni_1367 | MS-MS/MS | MA | 664 | 74.1938 | 74 | 7.91 | 328/48 | −/− | 0 | 0 | 2/1 |
| Igni_1383 | MS-MS/MS | Cyt | 740 | 82.9741 | 80 | 6.17 | 442/49 | −/− | 0 | 0 | 2/3 |
Cutoff value in all cases: 80; MS-MS/MS: combined research
n.s. Not shown, TMH transmembrane helix, SP signal peptide
aPutative subcellular localization, based on the fraction in which the protein was found, and on the annotated function: Cyt cytoplasmatic, M membrane, MA membrane associated, (P) periplasmic, E extracellular; these putative functions need to be verified experimentally
bN-terminal sequencing by Edman degradation