Table 1.
Primer sequences of the used housekeeping genes and genes of interest
| Genes | Accession member | Primer sequence 5′–3′ | Primer sequence 3′–5′ | Primer efficiency (%) |
|---|---|---|---|---|
| Nuclear-encoded reference genes | ||||
| 18S rRNA | AJ236016 | CCGGCGACGCATCATT | AGGCCACTATCCTACCATCGAA | 106.24 |
| Actin 9 | X69885 | CTATTCTCCGCTTTGGACTTGGCA | AGGACCTCAGGACAACGGAAACG | 95.67 |
| Elongation factor 1 | Z14079 | TTCTCGACTGCCACACTTCCA | TCCTTACCAGAACGCCTGTCAAT | 96.12 |
| Alfa-tubulin | AJ421412.1 | GATGTTGTGCCAAAGGATGTCA | GGCTGATAGTTGATACCACACTTGAAT | 93.43 |
| rbcS | X02353 | AATGGATGGGTTCCTTGTTT | GTATGCCTTCTTCGCCTCTC | 107.16 |
| Plastid-encoded reference genes | ||||
| 16S rRNA | V00165 | GCATGTGGTTTAATTCGATGCA | CCGAAGGCACCCCTCTCT | 104.15 |
| accD | Z00044 | CGAAAGGAATGGTGAAGTTGA | CTGCCAGGAGATAGAGTCAAAA | 98.50 |
| Initiation factor 1 | Z00044 | CGAAAGGAATGGTGAAGTTGA | CTGCCAGGAGATAGAGTCAAAA | 97.34 |
| ndhI | Z00044 | TTGGACATACACGCACACATAC | AATACGAGCCGCCAGATACA | 95.46 |
| ndhC | Z00044 | TTCCAATGCCCCCTTTC | ATGGGCGATGCTTGGTT | 90.45 |
| rps2 | Z00044 | TTCGGGAGACGGTTGAGT | GCAGCAAGTAGGGGAAAACA | 95.17 |
| rps3 | Z00044 | GGGGAACCCTACCTTCTCTG | CCGAAAACTGAACATTGCTG | 96.28 |
| rps11 | Z00044 | GCGGAGGACCAAGAAACTAC | TGGCAAAAGCTATACCGAAA | 88.85 |
| rpoC2 | Z00044 | GTTGTGCCCGAAAGGTTATG | TCTGTGAGTCCTCGGAATGG | 92.59 |
| Photosynthesis genes of interest | ||||
| Nuclear-encoded | ||||
| psbO | AY220076 | CGTGTGCCCTTCCTCTTCA | GATCCACCCCGTCCCTTT | 114.10 |
| atpC | X63606 | CCCCTCACCAAAGTAAGACC | GCCTGCGGATGAAATAAGA | 108.30 |
| Plastid-encoded | ||||
| petD | Z00044 | ATTGGTGAACCGGCAGA | GCTACTGGACGGCGAAA | 107.51 |
| psbE | Z00044 | TATTCATTGCGGGTTGGTT | ATTCCTTGTCGGCTCTCTGT | 111.88 |
| psaA | Z00044 | TGGCTTTGTTGCCTATTCC | CTCTTCCAGGTCCATCACAA | 113.28 |
| psaB | Z00044 | GCTTGGACAGGGCATTTAG | ACTACTTGAATCGGGGTTTTG | 107.59 |