Table 1.

Toxin neutralization and antigenic specificity of LT-binding VHHs

Clone	Vero cell assay IC75		GM1-LT ELISA IC50		Titer in ELISA (mg/L)
	Mean ± SDa (mg/L)		Mean ± SD (mg/L)		LT	CT	CTB	CTA
LT105	>10b	±0	>10	±0	0.073	1.3	0.26	>10
LT106	3.3	±0	0.028	±0.003	0.032	0.25	0.12	>10
LT109	0.0017	±0.0012	0.021	±0.008	0.18	0.12	0.15	>10
LT109 endoHc	0.0084	±0.005	0.06	±0.024	NDd	ND	ND	ND
LT128	>10	±0	>10	±0	0.002	0.098	0.027	>10
LT131	6.7	±4.7	>10	±0	0.023	0.023	0.012	>10
LT132	>10	±0	>10	±0	0.020	0.024	0.013	>10
LT141	3.3	±0	>10	±0	0.038	0.019	0.016	>10
LT143	>10	±0	>10	±0	0.028	0.040	0.016	>10
LT101	>10	±0	0.91	±0.34	0.042	4.6	1.6	>10
LT104	>10	±0	7.2	±4.9	0.010	>10	8.6	>10
LT111	1.1	±0	0.67	±0.26	0.042	3.1	0.83	>10
LT116	>10	±0	1.5	±1.4	0.010	0.66	0.21	>10
LT133	>10	±0	8.5	±6.4	0.059	4.1	1.6	>10
LT129	0.21	±0.23	0.81	±0.14	0.20	3.6	2.9	>10
LT139	>10	±0	0.18	±0.11	0.059	8.1	>10	>10
LT103	0.74	±0.52	0.86	±0.29	0.16	0.13	0.071	>10
LT124	0.12	±0	0.27	±0.11	0.054	0.037	0.033	>10
LT130	0.21	±0.23	0.78	±0.01	0.035	0.032	0.023	>10
LT154	0.39	±0.03	0.96	±1.02	0.048	0.022	0.016	>10
LT155	0.02	±0.009	0.21	±0.25	0.028	0.014	0.010	>10
LT156	0.17	±0.06	2.1	±2.5	0.024	0.019	0.010	>10
LT161	0.27	±0.21	2.9	±3.6	0.20	0.061	0.026	>10
LT163	0.07	±0.05	0.18	±0.03	0.038	0.026	0.024	>10
LT165	0.03	±0.01	0.09	±0.02	0.12	0.042	0.043	>10
LT176	1.1	±0.00	1.2	±0.39	0.040	0.030	0.023	>10
LT168	1.4	±0.39	0.03	±0.00	0.057	0.042	0.038	>10
LT170	0.39	±0.03	0.03	±0.01	0.073	0.048	0.038	>10

^aSD, standard deviation

^b>10, the VHH showed no effect at the highest concentration analyzed (10 mg/L)

^cendoH, sample that was deglycosylated by endoglycosidase H treatment

^dND, not determined