FIGURE 2.
Poly(glycerol phosphate) synthesis is linear with time and independent of polymer size. Reactions were conducted with 2 mm CDP-glycerol, 2 μm Lipid ϕ.1 analog, and 2.5 nm TagF, and reaction progress was determined by CMP formation via PIC-HPLC. The degree of Lipid ϕ analog polymerization at each time point was approximated as reported previously by the ratio of CDP-glycerol consumption to the initial concentration of the Lipid ϕ.1 analog (21). Inset, calculated data are shown. A 2-fold increase (- - -) and 2-fold decrease (· · ·) in Km are compared with a substrate with a constant (—) Km. The simulated data were generated using reaction conditions equivalent to those used in the experiment in which the substrate concentration was equivalent to the Km.