FIGURE 4.

Expression of ABCA1, ABCA7, and ABCC1 in rat erythrocytes. IOVs prepared from rat erythrocytes and membrane fractions from rat liver, platelet-rich plasma, and HEK293 cells expressing ABCA1, ABCA7, or ABCC1 were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane for Western blotting with anti-Na⁺-K⁺ ATPase mAb, anti-ABCA1 mAb, anti-ABCA7 mAb, and anti-ABCC1 mAb. Expression of the ubiquitous protein Na⁺-K⁺ ATPase was used for a loading control for each membrane fraction.