FIGURE 5.
Effect of phosphorylated sphingolipid on S1P transport into IOVs. IOVs were preincubated with 10 μm [33P]S1P in the presence of additional 100 μm cold S1P, 100 μm DHS1P, 50 μm ceramide 1-phosphate (C1P), or the absence of these compounds (con) at 37 °C for 5 min and then incubated in the presence or absence of 2 mm ATP at 37 °C for 5 min. S1P uptake was calculated as the difference between the S1P amounts in the presence and absence of ATP. The S1P uptake in the absence of compounds (con) is set at 100%. Experiments were performed three times, and the error bars indicate the S.D.