TABLE 1.
Bacterial strains and plasmids with relevant genotype and phenotype
| Strain or plasmid | Relevant genotype and characteristic(s) | Source or reference |
|---|---|---|
| Strain | ||
| S17-1λpir | Tpr SmrrecA thi hsdRM+ RP4 ::2-Tc ::Mu ::Km Tn7 λpir phage lysogen | 13 |
| M15 | Strain for regulated high level expression with pQE vectors | Qiagen |
| MC4100 | F−, araD319 Δ(argF-lac)U169 rpsL150(Smr) relA1 flbB5301 deoC1 ptsF25 rbsR | 14 |
| AFMC | MC4100 spontaneous rifampicin-resistant mutant (Rifr) | 15 |
| AFMCRAL | AFMC with chromosomal insertion mini-Tn5Km mhpR/Pa-lacZ | 9 |
| AFMCAL | AFMC with chromosomal insertion mini-Tn5Km Pa-lacZ | 9 |
| MC1061 | hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi mhpRABCD | 16 |
| ED1061 | MC1061 derivative with chromosomal insertion Km in hcaA1 | 8 |
| ED1061AL | ED1061 with chromosomal insertion mini-Tn5Sm/Sp Pa-lacZ | This work |
| Plasmid | ||
| pUC18 | Apr; oriColE1 high copy number cloning vector, lacZα | 16 |
| pPAL | Apr, pUC18 derivative harboring the mhpR gene | 9 |
| pSJ3 | Apr; lacZ promoter probe vector, lacZ fusion flanked by NotI sites | 17 |
| pAL | Apr; pSJ3 containing a 0.5-kilobase DNA fragment to produce the mhpR/Pa-lacZ fusion | 9 |
| pRAL | Apr; pSJ3 containing a 1.1-kilobase DNA fragment to produce the mhpR/Pa-lacZ fusion | 9 |
| pJCD01 | Apr; oriColE1, polylinker of pUC19 flanked by rpoC and rrnBT1T2 terminators | 18 |
| pJCDAR | pJCD01 derivative containing Pr-Pa promoters | This work |
| pREP4 | kmr, plasmid that expresses the lacI repressor | Qiagen |
| pQE32 | Apr, oriColE1 T5 promoter lac operator, λ to/E. coli rrnB T1 terminators, N-terminal His6 | Qiagen |
| pQMH | Apr, pQE32 derivative containing the His6-mhpR gene | This work |
| pUT miniTn5 Sm/Sp | Apr Smr; delivery plasmid for mini-Tn5 Sm/Sp | 19 |
| pUTAL-2 | Apr Smr, pUTminiTn5 Sm/Sp containing the 4.4-kilobase NotI Pa-lacZ fragment from pAL | This work |