FIGURE 8.

DC-SIGN ECD ab initio model. A, superimposition of the CRDs tetramer (PDB code 1k9i) and neck modules assumed to be arranged in coiled-coil over the GASBOR model. Domains and modules were manually positioned. The oligosaccharide moiety found in PDB 1k9i is shown using an orange CPK representation, although it was not present in our samples, solely to help visualize the interaction sites of the protein with carbohydrate ligands. B, comparison of CRDs arrangement in the DC-SIGN model derived from our SAXS data and in the crystal structure of a fragment of DC-SIGNR containing the four CRDs and two neck repeats (PDB code 1xar).