FIGURE 4.

CD2 and CD11a played important roles in NK92 cell cytotoxicity. A, expression of some adhesion molecules on NK92 cells. NK92 cells were stained with indicated mAbs and analyzed by flow cytometry. B, confocal microscopic observation of accumulation of CD2, CD11a, and CD11c at the interface of NK92-K562 interaction (400×). C, blockade of CD2 or/and CD11a affected NK92-K562 conjugate formation. NK92 cells were preincubated with 10 μg/ml mouse IgG, anti-CD2, anti-CD11a, or anti-CD2 + anti-CD11a for 30 min and were mixed with equal numbers of CFSE-labeled K562 at 37 °C for 15 min. Then the cells were harvested, fixed, stained with PE-CD56 mAb, and analyzed by flow cytometry. D, blockade of CD2 or/and CD11a affected NK92 cell cytotoxicity against K562 targets. NK92 cells were preincubated with 20 μg/ml mouse IgG, anti-CD2, anti-CD11a, or anti-CD2 + anti-CD11a for 30 min, then equal volumes of K562 cells were added for a 4-h ⁵¹Cr-release assay. For C and D, data of control group were taken as 100%, and the relative ratio of other groups against control were presented. Data were collected from three independent experiments and analyzed by Student's t test, *, p < 0.05; **, p < 0.01 compared with control.