FIGURE 4.
Identification of transcription initiation point in the P zwf, Pedd, and P gap-1 promoters. A, transcription mapping. P. putida cells were grown on M9 minimal medium with glucose, and RNA was extracted as described by Marqués et al. (48). Primer used for extension reactions are given under “Experimental Procedures.” Lanes A, C, G, and T are sequencing ladders. The left lanes show single-stranded DNA markers of the indicated size. B, sequence of the mapped promoters. The tsp is indicated by a letter in larger size and marked +1. Boxed DNA corresponds to protected sequences in footprint (see Fig. 6). Underneath sequences correspond to oligoprimers used for PCR amplification.