FIGURE 5.

Electrophoresis mobility shift assay of the promoter region of P _zwf and P_edd/gap-1 by purified HexR. A, the DNA binding assays was done using purified HexR with 2 nm concentrations of the product of PCR (280 bp) of P_zwf end-labeled with ³²P and incubated with the concentration of purified HexR indicated at the top of each lane. The position of the free DNA is indicated. B, as in A, but a 340-bp P_edd/gap-1 fragment was used. Complex I and Complex II are indicated. C and D, as in A, but a 280-bp DNA fragment containing the HexR binding site at P_gap-1 (D) or P_edd (C) were used. E, the densitometric analysis of the EMSA gels. Squares, P_zwf promoter fragments; circles, P_edd promoter fragments; triangles, and P_gap-1 promoter fragments.