FIGURE 1.
The actual D. radiodurans DdrB protein is shorter than indicated in GenBankTM. A, nucleotide sequence of ddrB showing the ribosome binding site eight nucleotides upstream of the second ATG in the annotated open reading frame. The three consecutive rare (in E. coli) arginine codons are underlined. B, alignment of the DdrB protein sequences from the three Deinococcus species sequenced to date. The actual DdrB sequence is in boldface. DR0070, D. radiodurans; Dgeo0295, D. geothermalis; and Deide02990, D. deserti. The numbers below each position are calculated by the JALview program and reflect the degree of conservation of physicochemical properties in each column; 0 denotes no conservation, 1 to 9 reflect increasing levels of conservation (high numbers reflect amino acid substitutions in the same physicochemical class), and * indicates perfect conservation.